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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2A All Species: 56.36
Human Site: Y270 Identified Species: 88.57
UniProt: P11388 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11388 NP_001058.2 1531 174385 Y270 P V K G F R S Y V D M Y L K D
Chimpanzee Pan troglodytes XP_516332 1634 184611 Y299 P V N G F R S Y V D L Y V K D
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 Y285 P V N G F R S Y V D L Y V K D
Dog Lupus familis XP_537646 1532 174610 Y270 P V K G F R S Y V D M Y L K D
Cat Felis silvestris
Mouse Mus musculus Q01320 1528 172859 Y269 P V K G F R S Y V D L Y L K D
Rat Rattus norvegicus P41516 1526 173202 Y268 P V K G F R S Y V D M Y L K D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519680 1746 195650 Y298 P V K G F R G Y V D L Y V K D
Chicken Gallus gallus O42130 1553 174974 Y271 P V K G F R S Y V D L Y L K D
Frog Xenopus laevis NP_001082502 1579 178601 Y268 P V S G F R T Y V E M Y L K D
Zebra Danio Brachydanio rerio NP_001003834 1574 177254 Y269 P I N N F R T Y V D L Y V K D
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 Y251 G V R N F K D Y I D L H I K N
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 Y305 P I K G F E D Y V Q M Y T S Q
Sea Urchin Strong. purpuratus XP_783546 1448 163750 Y295 P V N G F K S Y V D L Y I K D
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 Y272 P V K S F T D Y V D L Y L S A
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 Y250 K I R N F K N Y V E L Y L K S
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 67.2 67.5 93.6 N.A. 89 88.7 N.A. 71.3 76.3 70.7 67.9 N.A. 51.2 N.A. 53 58.9
Protein Similarity: 100 78.6 78.4 96.4 N.A. 93.6 93.2 N.A. 79.1 85 81.8 78.8 N.A. 66.8 N.A. 70.4 73.4
P-Site Identity: 100 80 80 100 N.A. 93.3 100 N.A. 80 93.3 80 60 N.A. 33.3 N.A. 53.3 73.3
P-Site Similarity: 100 93.3 93.3 100 N.A. 100 100 N.A. 93.3 100 93.3 86.6 N.A. 80 N.A. 60 93.3
Percent
Protein Identity: N.A. N.A. N.A. 45 42.2 N.A.
Protein Similarity: N.A. N.A. N.A. 61.4 60.4 N.A.
P-Site Identity: N.A. N.A. N.A. 60 40 N.A.
P-Site Similarity: N.A. N.A. N.A. 66.6 80 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 20 0 0 80 0 0 0 0 74 % D
% Glu: 0 0 0 0 0 7 0 0 0 14 0 0 0 0 0 % E
% Phe: 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 7 0 0 74 0 0 7 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 % H
% Ile: 0 20 0 0 0 0 0 0 7 0 0 0 14 0 0 % I
% Lys: 7 0 54 0 0 20 0 0 0 0 0 0 0 87 0 % K
% Leu: 0 0 0 0 0 0 0 0 0 0 67 0 54 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 34 0 0 0 0 % M
% Asn: 0 0 27 20 0 0 7 0 0 0 0 0 0 0 7 % N
% Pro: 87 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 7 0 0 0 0 7 % Q
% Arg: 0 0 14 0 0 67 0 0 0 0 0 0 0 0 0 % R
% Ser: 0 0 7 7 0 0 54 0 0 0 0 0 0 14 7 % S
% Thr: 0 0 0 0 0 7 14 0 0 0 0 0 7 0 0 % T
% Val: 0 80 0 0 0 0 0 0 94 0 0 0 27 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 100 0 0 0 94 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _